Anti-GRB10 Antibody Picoband®

About this product

Boster Bio Anti-GRB10 Antibody Picoband® catalog # A01663-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01663-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors (e.g., IGF1R and IGF2R) . Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms

Deoxycytidine kinase; dCK; DCK

Gene Name

GRB10

Gene ID

2887

UniProt

Q13322

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GRB10 recombinant protein (Position: M1-K251) .

Clonality

Polyclonal

Tissue Specificity

Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level) .

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Cancer, Cell Cycle, Kinases/Phosphatases, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA) . Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

References & Citations

1. Arnaud, P., Monk, D., Hitchins, M., Gordon, E., Dean, W., Beechey, C. V., Peters, J., Craigen, W., Preece, M., Stanier, P., Moore, G. E., Kelsey, G.Conserved methylation imprints in the human and mouse GRB10 genes with divergent allelic expression suggests differential reading of the same mark.Hum. Molec. Genet. 12: 1005-1019, 2003. 2. Charalambous, M., Smith, F. M., Bennett, W. R., Crew, T. E., Mackenzie, F., Ward, A.Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism.Proc. Nat. Acad. Sci. 100: 8292-8297, 2003. 3. Frantz, J. D., Giorgetti-Peraldi, S., Ottinger, E. A., Shoelson, S. E.Human GRB-IR-beta/GRB10: splice variants of an insulin and growth factor receptor-binding protein with PH and SH2 domains.J. Biol. Chem. 272: 2659-2667, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50514 MW

Observed Molecular Weight

76 kDa

Applications Notes

6

Gene Name Synonym

Deoxycytidine kinase

Subcellular Location

Nucleus.

Protein Name

Carboxypeptidase M

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.