Anti-SHANK3 Antibody Picoband®

About this product

Boster Bio Anti-SHANK3 Antibody Picoband® catalog # A01231-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01231-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH3 and multiple ankyrin repeat domains 3 (Shank3), also known as proline-rich synapse-associated protein 2 (ProSAP2), is a protein that in humans is encoded by the SHANK3 gene. This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Additionally, Shank proteins play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified.

Synonyms

Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC5; KIAA0600

Gene Name

SHANK3

Gene ID

85358

UniProt

Q9BYB0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SHANK3 recombinant protein (Position: E1391-H1698) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Acetylation, Cardiovascular, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Hypertrophy, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

References & Citations

1. Entrez Gene: SHANK3 SH3 and multiple ankyrin repeat domains 3. 2. Boeckers TM, Bockmann J, Kreutz MR, Gundelfinger ED (2002) . ProSAP/Shank proteins - a family of higher order organizing molecules of the postsynaptic absorbance with an emerging role in human neurological disease.J. Neurochem. 81 (5) : 903–10.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

180-190 kDa

Applications Notes

6

Gene Name Synonym

Histone deacetylase 5

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

Vinculin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.