Anti-MCPIP1/ZC3H12A Antibody Picoband®

About this product

Rabbit IgG polyclonal antibody for MCPIP1/ZC3H12A detection. Tested with WB, IHC, ICC/IF, Flow Cytometry, ELISA in Human; Mouse; Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01688-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Zinc finger CCCH-type containing 12A is a protein in humans that is encoded by the ZC3H12A gene. ZC3H12A is an MCP1 (CCL2; MIM 158105) -induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.

Synonyms

Periaxin; PRX; KIAA1620

Gene Name

ZC3H12A

Gene ID

80149

UniProt

Q5D1E8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MCPIP1/ZC3H12A recombinant protein (Position: Q36-T98) .

Clonality

Polyclonal

Tissue Specificity

Detected in spinal cord (PubMed:11133365) . Isoform 1 and isoform 2 are found in sciatic nerve and Schwann cells (PubMed:11157804) .

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Adapters, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells. Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli. Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions. Required for normal remyelination after nerve injury. Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses; shorter internodes cause slower transmission of nerve impulses. Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane; the Schwann cell cytoplasm is restricted to regions between these appositions. Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves. Recruits DRP2 to the Schwann cell plasma membrane. Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology.

References & Citations

1. Liang, J., Wang, J., Azfer, A., Song, W., Tromp, G., Kolattukudy, P. E., Fu, M. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J. Biol. Chem. 283: 6337-6346, 2008. 2. Lin, R.-J., Chu, J.-S., Chien, H.-L., Tseng, C.-H., Ko, P.-C., Mei, Y.-Y., Tang, W.-C., Kao, Y.-T., Cheng, H.-Y., Liang, Y.-C., Lin, S.-Y. MCPIP1 suppresses hepatitis C virus replication and negatively regulates virus-induced proinflammatory cytokine responses. J. Immun. 193: 4159-4168, 2014. 3. Matsushita, K., Takeuchi, O., Standley, D. M., Kumagai, Y., Kawagoe, T., Miyake, T., Satoh, T., Kato, H., Tsujimura, T., Nakamura, H., Akira, S. Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay. Nature 458: 1185-1190, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

73 kDa

Applications Notes

6

Gene Name Synonym

Periaxin

Subcellular Location

Isoform 1: Cell membrane.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.