Anti-CXCR2 Antibody Picoband®

About this product

Boster Bio Anti-CXCR2 Antibody Picoband® catalog # A00455-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00455-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine) . Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Synonyms

Neutrophil gelatinase-associated lipocalin; NGAL; Lipocalin-2; SV-40-induced 24P3 protein; Siderocalin LCN2; p25; Lcn2

Gene Name

CXCR2

Gene ID

3579

UniProt

P25025

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CXCR2 recombinant protein (Position: F32-I54; A177-R212) .

Clonality

Polyclonal

Tissue Specificity

Detected in lung, spleen, uterus, vagina and epididymis. .

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Immunoglobulins, Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .

References & Citations

1. Murphy P.M., Tiffany H.L.; Cloning of complementary DNA encoding a functional human interleukin-8 receptor.; Science 253:1280-1283 (1991) . 2. Cerretti D.P., Kozlosky C.J., Vanden Bos T., Nelson N., Gearing D.P., Beckmann M.P. Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2.; Mol. Immunol. 30:359-367 (1993) . 3. Sprenger H., Lloyd A.R., Lautens L.L., Bonner T.I., Kelvin D.J.; Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.; J. Biol. Chem. 269:11065-11072 (1994) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22875 MW

Observed Molecular Weight

41 kDa

Applications Notes

6

Gene Name Synonym

Neutrophil gelatinase-associated lipocalin

Subcellular Location

Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

Protein Name

Neutrophil gelatinase-associated lipocalin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.