Anti-DR1 Antibody Picoband®

About this product

Boster Bio Anti-DR1 Antibody Picoband® catalog # A02204-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02204-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein Dr1 is a protein that in humans is encoded by the DR1 gene. This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription.

Synonyms

Cone-rod homeobox protein; CRX; CORD2

Gene Name

DR1

Gene ID

1810

UniProt

Q01658

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DR1 recombinant protein (Position: N7-A154) .

Clonality

Polyclonal

Tissue Specificity

Retina.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Neuroscience, Sensory System, Transcription, Transcription Factors, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors. .

References & Citations

1. Goppelt, A., Stelzer, G., Lottspeich, F., Meisterernst, M. A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains. EMBO J. 15: 3105-3116, 1996. 2. Inostroza, J. A., Mermeistein, F. H., Ha, I., Lane, W. S., Reinberg, D. Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription. Cell 70: 477-489, 1992. 3. Kamada, K., Shu, F., Chen, H., Malik, S., Stelzer, G., Roeder, R. G., Meisterernst, M., Burley, S. K. Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex. Cell 106: 71-81, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32261 MW

Observed Molecular Weight

19 kDa

Applications Notes

6

Gene Name Synonym

Cone-rod homeobox protein

Subcellular Location

Nucleus .

Protein Name

Cone-rod homeobox protein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.