Anti-IL2 Receptor gamma/IL2RG Antibody Picoband®

About this product

Boster Bio Anti-IL2 Receptor gamma/IL2RG Antibody Picoband® catalog # A00308-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00308-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

IL2RG (INTERLEUKIN 2 RECEPTOR, GAMMA), also known as Common gamma chain or CD132, is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors:IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. IL2RG is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. By fluorescence in situ hybridization and PCR amplification of somatic cell hybrid DNAs, The IL2RG gene is mapped to Xq13.1. The IL2RG gene contains 8 exons and spans approximately 4.2 kb. And the gamma chain is an indispensable component of the functional IL2 receptor. Lymphocytes expressing the common gamma chain can form functional receptors for these cytokine proteins, which transmit signals from one cell to another and programs of cellular differentiation. The sharing of the gamma subunit by several receptors explains why humans and mice that lack IL2 entirely show milder symptoms than those with IL2RG deficiency.

Synonyms

Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1; IFNAR1; IFNAR

Gene Name

IL2RG

Gene ID

3561

UniProt

P31785

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human IL2 Receptor gamma/IL2RG, which shares 78.9% amino acid (aa) sequence identity with both mouse and rat IL2 Receptor gamma/IL2RG.

Clonality

Polyclonal

Tissue Specificity

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. .

Applications

IF, ICC, WB

Field of Research

Cancer, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interferons, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Tumor Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986) . Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986) . Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta- subunits themselves (PubMed:7665574, PubMed:21854986) . Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity) . .

References & Citations

1. Asao, H., Okuyama, C., Kumaki, S., Ishii, N., Tsuchiya, S., Foster, D., Sugamura, K. Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J. Immun. 167: 1-5, 2001. 2. Brandt, K., Singh, P. B., Bulfone-Paus, S., Ruckert, R. Interleukin-21: a new modulator of immunity, infection, and cancer. Cytokine Growth Factor Rev. 18: 223-232, 2007. 3. PDB 2B5IWang X, Rickert M, Garcia KC (November 2005) . Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors. Science 310 (5751) : 1159–63. doi:10.1126/science.1117893. PMID 16293754.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63525 MW

Observed Molecular Weight

65 kDa

Gene Name Synonym

Interferon alpha/beta receptor 1

Subcellular Location

Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .

Protein Name

Interferon alpha/beta receptor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.