Anti-MCU Antibody Picoband®

About this product

Boster Bio Anti-MCU Antibody Picoband® catalog # A00685-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00685-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The mitochondrial calcium uniporter (MCU) is a transmembrane protein that allows the passage of calcium ions from a cell's cytosol into mitochondria. Its activity is regulated by MICU1 and MICU2, which together with the MCU make up the mitochondrial calcium uniporter complex. This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants.

Synonyms

Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a; Serine/threonine-protein kinase; Endoribonuclease; ERN1

Gene Name

MCU

Gene ID

90550

UniProt

Q8NE86

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MCU recombinant protein (Position: V51-D351) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. High levels observed in pancreatic tissue.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

References & Citations

1. Baughman, J. M., Perocchi, F., Girgis, H. S., Plovanich, M., Belcher-Timme, C. A., Sancak, Y., Bao, X. R., Strittmatter, L., Goldberger, O., Bagorad, R. L., Koteliansky, V., Mootha, V. K. Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Nature 476: 341-345, 2011. 2. De Stefani, D., Raffaello, A., Teardo, E., Szabo, I., Rizzuto, R. A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature 476: 336-340, 2011. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 8/31/2011.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

129383 MW

Observed Molecular Weight

34 kDa

Applications Notes

6

Gene Name Synonym

Endoplasmic reticulum to nucleus signaling 1

Subcellular Location

Endoplasmic reticulum membrane.

Protein Name

Thrombospondin-1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.