Anti-GRX2/GLRX2 Antibody Picoband®

About this product

Boster Bio Anti-GRX2/GLRX2 Antibody Picoband® catalog # A05331. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05331

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

GLRX2 (Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins (e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al. (2001) and Gladyshev et al. (2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al. (2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon (exon 1B) located upstream from the first exon used by the GLRX2A transcript (exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al. (2001) assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.

Synonyms

Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097

Gene Name

GLRX2

Gene ID

51022

UniProt

Q9NS18

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GRX2/GLRX2 recombinant protein (Position: M1-Q164) .

Clonality

Polyclonal

Tissue Specificity

Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain; highly expressed in the Purkinje cell bodies of the cerebellum.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.

References & Citations

1. Gladyshev, V. N., Liu, A., Novoselov, S. V., Krysan, K., Sun, Q.-A., Kryukov, V. M., Kryukov, G. V., Lou, M. F.Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2.J. Biol. Chem. 276: 30374-30380, 2001. 2. Lilig, C. H., Berndt, C., Vergnolle, O., Lonn, M. E., Hudemann, C., Bill, E., Holmgren, A.Characterization of human glutaredoxin 2 as iron-sulfur protein: a possible role as redox sensor.Proc. Nat. Acad. Sci. 102: 8168-8173, 2005. 3. Lundberg, M., Johansson, C., Chandra, J., Enoksson, M., Jacobsson, G., Ljung, J., Johansson, M., Holmgren, A.Cloning and expression of a novel human glutaredoxin (Grx2) with mitochondrial and nuclear isoforms.J. Biol. Chem. 276: 26269-26275, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

18 kDa

Applications Notes

6

Gene Name Synonym

Cytoskeleton associated protein 5

Subcellular Location

Centrosome. Spindle pole. Spindle. Kinetochore.

Protein Name

Zinc finger protein Helios

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.