- Catalog number
- A00148
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
- Synonyms
- Angiogenin;3.1.27.-; Ribonuclease 5; RNase 5; ANG; RNASE5
- Gene Name
- IGF1
- Gene ID
- 3479
- UniProt
- P05019
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human IGF1 recombinant protein (Position: P50-A118) .
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .
- Applications
- ELISA (Cap), Flow Cytometry, IHC
- Field of Research
- Actin Binding Proteins, Actin, etc., Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton, Cytoskeleton/ECM, DNA/RNA, Epigenetics and Nuclear Signaling, Hypoxia, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Response To Hypoxia, RNA Processing, Signal Transduction, Translation
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) . Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .
- References & Citations
- 1. Höppener JW, de Pagter-Holthuizen P, Geurts van Kessel AH, Jansen M, Kittur SD, Antonarakis SE, Lips CJ, Sussenbach JS (1985) . The human gene encoding insulin-like growth factor I is located on chromosome 12. Hum. Genet. 69 (2) : 157–60. 2. Jansen M, van Schaik FM, Ricker AT, Bullock B, Woods DE, Gabbay KH, Nussbaum AL, Sussenbach JS, Van den Brande JL (1983) . Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306 (5943) : 609–11.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 16550 MW
- Observed Molecular Weight
- 25 kDa
- Applications Notes
- 6
- Gene Name Synonym
- Angiogenin
- Subcellular Location
- Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.
- Protein Name
- Angiogenin
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
