Anti-RGS6 Antibody Picoband®

About this product

Boster Bio Anti-RGS6 Antibody Picoband® catalog # A05753-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05753-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulator of G-protein signaling 6 is a protein that in humans is encoded by the RGS6 gene. This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known.

Synonyms

Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; PPP1R14A; CPI17, PPP1INL

Gene Name

RGS6

Gene ID

9628

UniProt

P49758

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human RGS6 recombinant protein (Position: D7-E357) .

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

References & Citations

1. Berman, D. M., Wang, Y., Liu, Z., Dong, Q., Burke, L.-A., Liotta, L. A., Fisher, R., Wu, X. A functional polymorphism in RGS6 modulates the risk of bladder cancer. Cancer Res. 64: 6820-6826, 2004. 2. Chatterjee, T. K., Liu, Z., Fisher, R. A. Human RGS6 gene structure, complex alternative splicing, and role of N terminus and G protein gamma-subunit-like (GGL) domain in subcellular localization of RGS6 splice variants. J. Biol. Chem. 278: 30261-30271, 2003. 3. Posner, B. A., Gilman, A. G., Harris, B. A. Regulators of G protein signaling 6 and 7: purification of complexes with G-beta-5 and assessment of their effects on G protein-mediated signaling pathways. J. Biol. Chem. 274: 31087-31093, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16693 MW

Observed Molecular Weight

54 kDa

Applications Notes

6

Gene Name Synonym

Protein phosphatase 1 regulatory subunit 14A

Subcellular Location

Cytoplasm .

Protein Name

Protein phosphatase 1 regulatory subunit 14A

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.