Anti-SOCS3 Antibody Picoband®

About this product

Boster Bio Anti-SOCS3 Antibody Picoband® catalog # A00274-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00274-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SOCS3 (Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN) -gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Synonyms

Agouti-related protein; Agrp; Agrt; Art

Gene Name

SOCS3

Gene ID

9021

UniProt

O14543

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SOCS3 recombinant protein (Position: S13-L225) .

Clonality

Polyclonal

Tissue Specificity

Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Calcium Signaling, Cancer, Cardiovascular, Endocrine System, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity) . It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity) .

References & Citations

1. Babon, J. J., McManus, E. J., Yao, S., DeSouza, D. P., Mielke, L. A., Sprigg, N. S., Willson, T. A., Hilton, D. J., Nicola, N. A., Baca, M., Nicholson, S. E., Norton, R. S.The structure of SOCS3 reveals the basis of the extended SH2 domain function and identifies an unstructured insertion that regulates stability.Molec. Cell 22: 205-216, 2006. 2. Chen, Z., Laurence, A., Kanno, Y., Pacher-Zavisin, M., Zhu, B.-M., Tato, C., Yoshimura, A., Hennighausen, L., O'Shea, J. J.Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells.Proc. Nat. Acad. Sci. 103: 8137-8142, 2006. 3. Jo, D., Liu, D., Yao, S., Collins, R. D., Hawiger, J.Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis.Nature Med. 11: 892-898, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68762 MW

Observed Molecular Weight

28-30 kDa

Applications Notes

6

Gene Name Synonym

Agouti related neuropeptide

Subcellular Location

Secreted.

Protein Name

Aryl hydrocarbon receptor nuclear translocator-like protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.