Anti-N WASP/WASL Antibody Picoband®

About this product

Boster Bio Anti-N WASP/WASL Antibody Picoband® catalog # A05438-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05438-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

Synonyms

POU domain, class 4, transcription factor 1

Gene Name

WASL

Gene ID

8976

UniProt

O00401

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211) .

Clonality

Polyclonal

Tissue Specificity

Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Applications

ELISA, Flow Cytometry, WB

Field of Research

Cell Type Marker, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain) . Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting ly with a highly conserved autoregulatory domain surrounding the transcription initiation site.

References & Citations

1. Cotta-de-Almeida, V., Westerberg, L., Maillard, M. H., Onaldi, D., Wachtel, H., Meelu, P., Chung, U., Xavier, R., Alt, F. W., Snapper, S. B. Wiskott-Aldrich syndrome protein (WASP) and N-WASP are critical for T cell development. Proc. Nat. Acad. Sci. 104: 15424-15429, 2007. 2. Fukuoka, M., Miki, H., Takenawa, T. Identification of N-WASP homologs in human and rat brain. Gene 196: 43-48, 1997. 3. Guenin-Mace, L., Veyron-Churlet, R., Thoulouze, M.-I., Romet-Lemonne, G., Hong, H., Leadlay, P. F., Danckaert, A., Ruf, M.-T., Mostowy, S., Zurzolo, C., Bousso, P., Chretien, F., Carlier, M.-F., Demangel, C. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. J. Clin. Invest. 123: 1501-1512, 2013.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

70 kDa

Applications Notes

6

Gene Name Synonym

POU class 4 homeobox 1

Subcellular Location

Nucleus.

Protein Name

Zinc finger protein Helios

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.