- Catalog number
- A00276-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
- Synonyms
- Agouti-related protein; Agrp; Agrt; Art
- Gene Name
- Epcam
- Gene ID
- 17075
- UniProt
- Q99JW5
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- Mouse EpCAM
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.
- Applications
- ELISA, IF, IHC
- Field of Research
- Calcium Signaling, Cancer, Cardiovascular, Endocrine System, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Signaling Pathway
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Function
- Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity) . It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity) .
- References & Citations
- 1. Litvinov, Sergey; et al. (1994) . Ep-CAM: a human epithelial antigen is a homophilic cell–cell adhesion molecule. The Journal of Cell Biology 125 (2) : 437–46. 2. Maetzel, Dorothea; et al. (2009) . Nuclear signalling by tumour-associated antigen EpCAM. Nature Cell Biology 11 (2) : 162–71.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 68762 MW
- Observed Molecular Weight
- 87 kDa
- Gene Name Synonym
- Agouti related neuropeptide
- Subcellular Location
- Secreted.
- Protein Name
- Aryl hydrocarbon receptor nuclear translocator-like protein 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
