Pyruvate Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F058-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Fluorescence method

Field of Research

Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle

Assay Principle

Pyruvate is an important component of the organism. It mainly participates in the metabolism of sugar and fat, and is also one of the intermediate products of carbohydrate metabolism. The detection principle of this kit is that pyruvate undergoes a series of reactions under the action of enzyme reagents, and the resulting substance can react with the color developer. The content of pyruvate in the sample is calculated by measuring the fluorescence value of excitation wavelength 535 nm and emission wavelength 587 nm.

Assay Performance Time

40 min

Sample Type

Serum; Plasma; Urine; Animal tissue; Cell

Detection Range

1.00-100 μmol/L

Sensitivity

1.00 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

2.0-4.0%

Intra CV

1.0-2.0%

Detection Instrument

Fluorescence microplate reader