Anti-Tigit Antibody Picoband®

About this product

Boster Bio Anti-Tigit Antibody Picoband® catalog # A01962-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01962-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is an immune receptor present on some T cells and Natural Killer Cells (NK) . This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH) . The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.

Synonyms

Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; IGFBP5; IBP5

Gene Name

Tigit

Gene ID

100043314

UniProt

P86176

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Tigit recombinant protein (Position: T29-G249) .

Clonality

Polyclonal

Tissue Specificity

Osteosarcoma, and at lower levels in liver, kidney and brain.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 6/16/2009. 2. Stanietsky, N., Simic, H., Arapovic, J., Toporik, A., Levy, O., Novik, A., Levine, Z., Beiman, M., Dassa, L., Achdout, H., Stern-Ginossar, N., Tsukerman, P., Jonjic, S., Mandelboim, O. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc. Nat. Acad. Sci. 106: 17858-17863, 2009. 3. Yu, X., Harden, K., Gonzalez, L. C., Francesco, M., Chiang, F., Irving, B., Tom, I., Ivelja, S., Refino, C. J., Clark, H., Eaton, D., Grogan, J. L. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nature Immun. 10: 48-57, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30570 MW

Observed Molecular Weight

30 kDa

Applications Notes

6

Gene Name Synonym

Insulin-like growth factor-binding protein 5

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.