Anti-UBE2D1/2/3/4 Antibody Picoband®

About this product

Boster Bio Anti-UBE2D1/2/3/4 Antibody Picoband® catalog # A04728-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04728-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Homeobox protein Hox-B1; Homeobox protein Hox-2I; HOXB1; HOX2I

Gene Name

UBE2D1/2/3/4

Gene ID

7321/7322/7323/51619

UniProt

P51668

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147) .

Clonality

Polyclonal

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures.

References & Citations

1. Gehrke, S. G., Riedel, H.-D., Herrmann, T., Hadaschik, B., Bents, K., Veltkamp, C., Stremmel, W. UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood 101: 3288-3293, 2003. 2. Gutierrez, J. A., Yu, J., Rivera, S., Wessling-Resnick, M. Functional expression cloning and characterization of SFT, a stimulator of Fe transport. J. Cell Biol. 139: 895-905, 1997. Note: Erratum: J. Cell Biol. 147: 205 only, 1999. 3. Gutierrez, J. A., Yu, J., Wessling-Resnick, M. Characterization and chromosomal mapping of the human gene for SFT, a stimulator of Fe transport. Biochem. Biophys. Res. Commun. 253: 739-742, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32193 MW

Observed Molecular Weight

17 kDa

Applications Notes

6

Gene Name Synonym

Ubiquitin conjugating enzyme E2 D1/2/3/4

Subcellular Location

Nucleus.

Protein Name

Ubiquitin-conjugating enzyme E2 D1/2/3/4

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.