- Catalog number
- A06172-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- YTH domain-containing protein 1 is a protein that in humans is encoded by the YTHDC1 gene. YTHDC1 is a nuclear protein involved in splice site selection that localises to YT bodies; dynamic subnuclear compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis.
- Synonyms
- CD160 antigen; Natural killer cell receptor BY55; CD160; BY55
- Gene Name
- YTHDC1
- Gene ID
- 91746
- UniProt
- Q96MU7
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human YTHDC1 recombinant protein (Position: S326-K651) .
- Clonality
- Polyclonal
- Tissue Specificity
- Expression is restricted to functional NK and cytotoxic T lymphocytes. Expressed in viral-specific effector memory and terminally differentiated effector memory CD8+ T cells. Expressed in memory and activated CD4+ T cell subsets. Expressed at high levels in intraepithelial lymphocytes. Expressed in both alpha-beta and gamma-delta CD8+ T cell subsets. Expressed in umbilical vein endothelial cells. Expressed in monocytes and at lower levels in B cells. Isoform 3: Expressed exclusively in activated NK cells.
- Applications
- WB, ELISA
- Field of Research
- Cancer, Cell Biology, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Function
- CD160 antigen: Receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells. Receptor for both classical and non-classical MHC class I molecules. In the context of acute viral infection, recognizes HLA-C and triggers NK cell cytotoxic activity, likely playing a role in anti-viral innate immune response. On CD8+ T cells, binds HLA-A2-B2M in complex with a viral peptide and provides a costimulatory signal to activated/memory T cells. Upon persistent antigen stimulation, such as occurs during chronic viral infection, may progressively inhibit TCR signaling in memory CD8+ T cells, contributing to T cell exhaustion. On endothelial cells, recognizes HLA-G and controls angiogenesis in immune privileged sites. Receptor or ligand for TNF superfamily member TNFRSF14, participating in biional cell-cell contact signaling between antigen presenting cells and lymphocytes. Upon ligation of TNFRSF14, provides stimulatory signal to NK cells enhancing IFNG production and anti-tumor immune response. On activated CD4+ T cells, interacts with TNFRSF14 and downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response. In the context of bacterial infection, acts as a ligand for TNFRSF14 on epithelial cells, triggering the production of antimicrobial proteins and proinflammatory cytokines. CD160 antigen, soluble form: The soluble GPI-cleaved form, usually released by activated lymphocytes, might play an immune regulatory role by limiting lymphocyte effector functions.
- References & Citations
- 1. Hartmann, A. M., Nayler, O., Schwaiger, F. W., Obermeier, A., Stamm, S. The interaction and colocalization of Sam68 with the splicing-associated factor YT521-B in nuclear dots is regulated by the Src family kinase p59 (fyn) . Molec. Biol. Cell 10: 3909-3926, 1999. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 01/03/2017. 3. Haussmann, I. U., Bodi, Z., Sanchez-Moran, E., Mongan, N. P., Archer, N., Fray, R. G., Soller, M. m (6) A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination. Nature 540: 301-304, 2016.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 85 kDa
- Applications Notes
- Western blot, 0.25-0.5μg/mL, Human, Mouse, Rat<br>
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/mL, Human, Rat<br>
Immunocytochemistry/Immunofluorescence, 5μg/mL, Human<br>
Immunoprecipitation, 0.5-2 μg/mL, Human<br>
ELISA, 0.1-0.5μg/mL, -<br>
- Gene Name Synonym
- CD160 molecule
- Subcellular Location
- Cell membrane. GPI-anchor. Secreted.
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
