Anti-ch TOG/CKAP5 Antibody Picoband® (monoclonal, 3C13)

About this product

Boster Bio Anti-ch TOG/CKAP5 Antibody Picoband® (monoclonal, 3C13) catalog # M05324. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M05324

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. It is mapped to 11p11.2. This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for ional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interact ernatively spliced transcript variants encoding different isoforms have been identified.

Synonyms

3-oxo-5-beta-steroid 4-dehydrogenase; Aldo-keto reductase family 1 member D1; Delta (4) -3-ketosteroid 5-beta-reductase; Delta (4) -3-oxosteroid 5-beta-reductase; AKR1D1; SRD5B1

Gene Name

CKAP5

Gene ID

9793

UniProt

Q14008

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221) .

Clonality

Monoclonal

Clone

Clone: 3C13

Tissue Specificity

Highly expressed in liver. Expressed in testis and weakly in colon.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta4-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-4-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one) .

References & Citations

1.Entrez Gene: CKAP5 cytoskeleton associated protein 5. 2.Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996) . Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors. Eur. J. Biochem. 234 (2) : 406–13.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

225 kDa

Applications Notes

6

Gene Name Synonym

Aldo-keto reductase family 1 member D1

Subcellular Location

Cytoplasm.

Protein Name

RNA-binding protein Musashi homolog 1

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.