Anti-ASS1 Antibody Picoband® (monoclonal, 5I5)

About this product

Boster Bio Anti-ASS1 Antibody Picoband® (monoclonal, 5I5) catalog # M02212-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02212-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. It is mapped to 9q34.11. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.

Synonyms

Adenosine kinase; AK; Adenosine 5'-phosphotransferase; ADK

Gene Name

ASS1

Gene ID

445

UniProt

P00966

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ASS1 recombinant protein (Position: S3-S365) .

Clonality

Monoclonal

Clone

Clone: 5I5

Tissue Specificity

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Ribosome, RNA Processing, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

References & Citations

1. Engel, K., Hohne, W., Haberle, J. Mutations and polymorphisms in the human argininosuccinate synthetase (ASS1) gene. Hum. Mutat. 30: 300-307, 2009. 2. Rabinovich, S., Adler, L., Yizhak, K., Sarver, A., Silberman, A., Agron, S., Stettner, N., Sun, Q., Brandis, A., Helbling, D., Korman, S., Itzkovitz, S., Dimmock, D., Ulitsky, I., Nagamani, S. C. S., Ruppin, E., Erez, A. Diversion of aspartate in ASS1-deficient tumours fosters de novo pyrimidine synthesis. Nature 527: 379-383, 2015. 3. Sase, M., Kobayashi, K., Imamura, Y., Saheki, T., Nakano, K., Miura, S., Mori, M. Level of translatable messenger RNA coding for argininosuccinate synthetase in the liver of the patients with quantitative-type citrullinemia. Hum. Genet. 69: 130-134, 1985.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

47 kDa

Applications Notes

6

Gene Name Synonym

Adenosine kinase

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

Mitofusin-1

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.