Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6)

About this product

Boster Bio Anti-eRF1/ETF1 Antibody Picoband® (monoclonal, 3B6) catalog # M04157. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M04157

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in ing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD) . Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

Synonyms

AW546468 antibody; C80305 antibody; MGC18200 antibody; MGC4569 antibody; MGC54054 antibody; OTTHUMP00000147628 antibody; OTTHUMP00000229864 antibody; OTTHUMP00000229865 antibody; OTTHUMP00000229866 antibody; OTTHUMP00000229870 antibody; OTTHUMP00000229871 antibody; OTTHUMP00000229872 antibody; Placental ribonuclease inhibitor antibody; Placental RNase inhibitor antibody; PRI antibody; RAI antibody; RI antibody; Ribonuclease inhibitor antibody; Ribonuclease/angiogenin inhibitor 1 antibody; Ribonuclease/angiogenin inhibitor antibody; RINI_HUMAN antibody; RNase inhibitor antibody; RNH 1 antibody; RNH antibody; RNH1 antibody; Rnh1 ribonuclease/angiogenin inhibitor 1 antibody

Gene Name

ETF1

Gene ID

2107

UniProt

P62495

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342) .

Clonality

Monoclonal

Clone

Clone: 3B6

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

References & Citations

1. Frolova, L., Le Goff, X., Rasmussen, H. H., Cheperegin, S., Drugeon, G., Kress, M., Arman, I., Haenni, A.-L., Celis, J. E., Philippe, M., Justesen, J., Kisselev, L. A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor. Nature 372: 701-703, 1994. 2. Guenet, L., Henry, C., Toutain, B., Dubourg, C., Le Gall, J. Y., David, V., Le Treut, A. Eukaryotic translation termination factor gene (ETF1/eRF1) maps at D5S500 in a commonly deleted region of chromosome 5q31 in malignant myeloid diseases. Cytogenet. Cell Genet. 88: 82-86, 2000. 3. Hansen, L. L., Jakobsen, C. G., Justesen, J. Assignment of the human translation termination factor 1 (ETF1) to 5q31.1 and of the proximal marker D5S1995 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 256-257, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

49 kDa

Applications Notes

6

Gene Name Synonym

Ribonuclease/angiogenin inhibitor 1

Subcellular Location

Cytoplasm.

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.