Anti-APPL/APPL1 Antibody Picoband® (monoclonal, 5G11)

About this product

Boster Bio Anti-APPL/APPL1 Antibody Picoband® (monoclonal, 5G11) catalog # M02381. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02381

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

DCC-interacting protein 13-alpha (APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54% amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.

Synonyms

ATP-citrate synthase; ATP-citrate (pro-S-) -lyase; ACL; Citrate cleavage enzyme; ACLY

Gene Name

APPL1

Gene ID

26060

UniProt

Q9UKG1

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human APPL/APPL1 recombinant protein (Position: K91-R668) .

Clonality

Monoclonal

Clone

Clone: 5G11

Tissue Specificity

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Applications

WB, IHC, Flow Cytometry

Field of Research

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

References & Citations

1. Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (Oct 1999) . Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2. Oncogene 18 (35) : 4891–8. doi:10.1038/sj.onc.1203080. PMID 10490823. 2. Nechamen CA, Thomas RM, Dias JA (Nov 2006) . APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex. Mol Cell Endocrinol 260-262: 93–9. doi:10.1016/j.mce.2006.08.014.PMC 1782224. PMID 17030088. 3. Entrez Gene: APPL1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

85 kDa

Applications Notes

6

Gene Name Synonym

ATP citrate lyase

Subcellular Location

Cytoplasm.

Protein Name

Mitofusin-1

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.