Anti-Nefh Antibody Picoband®

About this product

Boster Bio Anti-Nefh Antibody Picoband® catalog # A05307. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05307

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Synonyms

Loricrin; LOR; LRN

Gene Name

Nefh

Gene ID

380684

UniProt

P19246

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Nefh recombinant protein (Position: Y109-E466) .

Clonality

Polyclonal

Tissue Specificity

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Cell Biology, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interferons, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Major keratinocyte cell envelope protein.

References & Citations

1. Lees, J. F., Shneidman, P. S., Skuntz, S. F., Carden, M. J., Lazzarini, R. A. The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988. 2. Mattei, M.-G., Dautigny, A., Pham-Dinh, D., Passage, E., Mattei, J.-F., Jolles, P. The gene encoding the large human neurofilament subunit (NF-H) maps to the q121-q131 region on human chromosome 22. Hum. Genet. 80: 293-295, 1988. 3. Rao, M. V., Houseweart, M. K., Williamson, T. L., Crawford, T. O., Folmer, J., Cleveland, D. W. Neurofilament-dependent radial growth of motor axons and axonal organization of neurofilaments does not require the neurofilament heavy subunit (NF-H) or its phosphorylation. J. Cell Biol. 143: 171-181, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

117-220 kDa

Applications Notes

6

Gene Name Synonym

Loricrin

Subcellular Location

Nucleoplasm. Cytoplasm.

Protein Name

Zinc finger protein Helios

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.