Anti-MPI Picoband® Antibody (monoclonal, 5G5)

About this product

Boster Bio Anti-MPI Picoband® Antibody (monoclonal, 5G5) catalog # M00175-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00175-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P) . It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.

Synonyms

Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; PMI; MPI; PMI1

Gene Name

MPI

Gene ID

4351

UniProt

P34949

Host

Mouse

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MPI recombinant protein (Position: A2-K99) . Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.

Clonality

Monoclonal

Clone

Clone: 5G5

Tissue Specificity

Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Channels, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

References & Citations

1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998. 2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib) . Hum. Mutat. 16: 247-252, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46659 MW

Observed Molecular Weight

45 kDa

Applications Notes

6

Gene Name Synonym

Mannose phosphate isomerase

Subcellular Location

Cytoplasm

Protein Name

Isocitrate dehydrogenase [NADP] cytoplasmic

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.