Anti-DDX1 Picoband® Antibody (monoclonal, 3I10)

About this product

Boster Bio Anti-DDX1 Picoband® Antibody (monoclonal, 3I10) catalog # M03727. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M03727

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Synonyms

Eukaryotic translation initiation factor 6; eIF-6; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP) ; EIF6; EIF3A; ITGB4BP; OK/SW-cl.27

Gene Name

DDX1

Gene ID

1653

UniProt

Q92499

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DDX1 recombinant protein (Position: K562-F740) .

Clonality

Monoclonal

Clone

Clone: 3I10

Tissue Specificity

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1) -dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC) . Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.

References & Citations

1. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002) . An RNA helicase, DDX1, interacting with poly (A) RNA and heterogeneous nuclear ribonucleoprotein K. The Journal of Biological Chemistry. 277 (43) : 40403–9. 2. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002) . Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5. The Journal of Biological Chemistry. 277 (44) : 41489–96. 3. Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006) . Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions. Cell Research. 15 (11–12) : 923–34.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

86 kDa

Applications Notes

6

Gene Name Synonym

Eukaryotic translation initiation factor 6

Subcellular Location

Nucleolus. Cytoplasm

Protein Name

Mediator of RNA polymerase II transcription subunit 15

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.