Anti-Tubulin alpha Picoband® Antibody (monoclonal, 7B12)

About this product

Boster Bio Anti-Tubulin alpha Picoband® Antibody (monoclonal, 7B12) catalog # M03989-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M03989-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2, UBLE1B; HRIHFB2115

Gene Name

TUBA1A/TUBA1B/TUBA1C

Gene ID

7846/10376/84790

UniProt

Q71U36

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Tubulin alpha recombinant protein (Position: N18-A403) .

Clonality

Monoclonal

Clone

Clone: 7B12

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

References & Citations

1. Aiken, J., Moore, J. K., Bates, E. A. TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity. Hum. Molec. Genet. 28: 1227-1243, 2019. 2. Bahi-Buisson, N., Poirier, K., Boddaert, N., Saillour, Y., Castelnau, L., Philip, N., Buyse, G., Villard, L., Joriot, S., Marret, S., Bourgeois, M., Van Esch, H., Lagae, L., Amiel, J., Hertz-Pannier, L., Roubertie, A., Rivier, F., Pinard, J. M., Beldjord, C., Chelly, J. Refinement of cortical dysgeneses spectrum associated with TUBA1A mutations. J. Med. Genet. 45: 647-653, 2008. 3. Cowan, N. J., Dobner, P. R., Fuchs, E. V., Cleveland, D. W. Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

56 kDa

Applications Notes

6

Gene Name Synonym

Ubiquitin like modifier activating enzyme 2

Subcellular Location

Nucleus; Cytoplasm

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.