Anti-BCL3 Antibody Picoband®

About this product

Boster Bio Anti-BCL3 Antibody Picoband® catalog # A02773-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02773-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. The BCL3 gene is mapped to human chromosome 19. This protein functions as a transcriptional coactivator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.

Synonyms

CD3 antigen, epsilon polypeptide; CD3e molecule; Cd3e; Cd3e_predicted; rCG_58471

Gene Name

BCL3

Gene ID

602

UniProt

P20749

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BCL3 recombinant protein (Position: E118-Y307) .

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Field of Research

Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501) . Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

References & Citations

1. Wulczyn FG, Naumann M, Scheidereit C (Sep 1992) . Candidate proto-oncogene bcl-3 encodes a subunit-specific inhibitor of transcription factor NF-kappa B. Nature 358 (6387) : 597–9. 2. Ohno H, Takimoto G, McKeithan TW (May 1990) . The candidate proto-oncogene bcl-3 is related to genes implicated in cell lineage determination and cell cycle control. Cell 60 (6) : 991–7. 3. McKeithan, T. W., Rowley, J. D., Shows, T. B., Diaz, M. O.Cloning of the chromosome translocation breakpoint junction of the t (14;19) in chronic lymphocytic leukemia.Proc. Nat. Acad. Sci. 84: 9257-9260, 1987.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

12252 MW

Observed Molecular Weight

60 kDa

Applications Notes

6

Gene Name Synonym

CD3e molecule

Subcellular Location

Alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; cell body; cell-cell junction; dendritic spine; integral component of membrane

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.