Anti-CD5 Antibody Picoband® (monoclonal, 11B4)

About this product

Boster Bio Anti-CD5 Antibody Picoband® (monoclonal, 11B4) catalog # M00480-4. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00480-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1

Gene Name

Cd5

Gene ID

54236

UniProt

Q4KLZ9

Host

Mouse

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat CD5 recombinant protein (Position: Q24-L494) .

Clonality

Monoclonal

Clone

Clone: 11B4

Tissue Specificity

Brain, liver, placenta, lymphocytes and erythrocytes.

Applications

WB, IHC

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May act as a receptor in regulating T-cell proliferation.

References & Citations

1. Lundell, A.-C., Johansen, S., Adlerberth, I., Wold, A. E., Hesselmar, B., Rudin, A. High proportion of CD5+ B cells in infants predicts development of allergic disease. J. Immun. 193: 510-518, 2014. 2. Zhang, C., Xin, H., Zhang, W., Yazaki, P. J., Zhang, Z., Le, K., Li, W., Lee, H., Kwak, L., Forman, S., Jove, R., Yu, H. CD5 binds to interleukin-6 and induces a feed-forward loop with the transcription factor STAT3 in B cells to promote cancer. Immunity 44: 913-923, 2016.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

67 kDa

Gene Name Synonym

CD5 molecule

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Protein Name

Integrin beta-2

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.