Anti-HP1 alpha/CBX5 Antibody Picoband® (monoclonal, 8G6)

About this product

Boster Bio Anti-HP1 alpha/CBX5 Antibody Picoband® (monoclonal, 8G6) catalog # M02780-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02780-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Synonyms

N-myc-interactor; Nmi; N-myc and STAT interactor; NMI

Gene Name

CBX5

Gene ID

23468

UniProt

P45973

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human HP1 alpha/CBX5 recombinant protein (Position: M1-S191) .

Clonality

Monoclonal

Clone

Clone: 8G6

Tissue Specificity

Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Motor Proteins, Myosin, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5.

References & Citations

1. Allan, R. S., Zueva, E., Cammas, F., Schreiber, H. A., Masson, V., Belz, G. T., Roche, D., Maison, C., Quivy, J.-P., Almouzni, G., Amigorena, S. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature 487: 249-253, 2012. 2. Bannister, A. J., Zegerman, P., Partridge, J. F., Miska, E. A., Thomas, J. O., Allshire, R. C., Kouzarides, T. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410: 120-124, 2001. 3. Cheutin, T., McNairn, A. J., Jenuwein, T., Gilbert, D. M., Singh, P. B., Misteli, T. Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science 299: 721-725, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

22 kDa

Applications Notes

6

Gene Name Synonym

N-myc (and STAT) interactor

Subcellular Location

Cytoplasm.

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.