Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)

About this product

Boster Bio Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) catalog # M02833-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02833-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Elongator complex protein 3, also named KAT9, is aproteinthat in humans is encoded by theELP3gene. It is mapped to 8p21.1. ELP3 is the catalytichistone acetyltransferasesubunit of theRNA polymeraseII elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzymeand is involved in transcriptional elongation. ELP3 supports the migration and branching ofprojection neuronsthroughacetylationof alpha-tubulin in the developingcerebral cortex. In mammals, ELP3 is important for paternalDNA demethylationafterfertilization.ELP3 is potentially involved in cellular redoxhomeostasisby mediating the acetylation ofglucose-6-phosphate dehydrogenase. Besides, ELP3 may play a role inchromatin remodelingand is involved in acetylation ofhistones H3and probablyH4.

Synonyms

Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6; STFB

Gene Name

ELP3

Gene ID

55140

UniProt

Q9H9T3

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human KAT9/ELP3, identical to the related mouse sequence.

Clonality

Monoclonal

Clone

Clone: 8B4

Tissue Specificity

Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Applications

WB

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

References & Citations

1. Hawkes NA, Otero G, Winkler GS, Marshall N, Dahmus ME, Krappmann D, Scheidereit C, Thomas CL, Schiavo G, Erdjument-Bromage H, Tempst P, Svejstrup JQ (Jan 2002) . Purification and characterization of the human elongator complex. J Biol Chem. 277 (4) : 3047–52. 2. Creppe C, Malinouskaya L, Volvert ML, Gillard M, Close P, Malaise O, Laguesse S, Cornez I, Rahmouni S, Ormenese S, Belachew S, Malgrange B, Chapelle JP, Siebenlist U, Moonen G, Chariot A, Nguyen L (Feb 2009) . Elongator controls the migration and differentiation of cortical neurons through acetylation of alpha-tubulin. Cell. 136 (3) : 551–64.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

62 kDa

Gene Name Synonym

Cystatin B (stefin B)

Subcellular Location

Cytoplasm

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.