Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2)

About this product

Boster Bio Anti-Integrin beta 5/ITGB5 Antibody Picoband® (monoclonal, 9E2) catalog # M04201. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M04201

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.

Synonyms

Heat shock protein 105 kDa; Antigen NY-CO-25; Heat shock 110 kDa protein; HSPH1; HSP105; HSP110; KIAA0201

Gene Name

ITGB5

Gene ID

3693

UniProt

P18084

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Integrin beta 5/ITGB5 recombinant protein (Position: S32-D689) .

Clonality

Monoclonal

Clone

Clone: 9E2

Tissue Specificity

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Applications

WB, IHC, Flow Cytometry

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.

References & Citations

1. Busk, M., Pytela, R., Sheppard, D. Characterization of the integrin alpha-v-beta-6 as a fibronectin-binding protein. J. Biol. Chem. 267: 5790-5796, 1992. 2. McLean, J. W., Vestal, D. J., Cheresh, D. A., Bodary, S. C. cDNA sequence of the human integrin beta 5 subunit. J. Biol. Chem. 265: 17126-17131, 1990. 3. Reynolds, L. E., Wyder, L., Lively, J. C., Taverna, D., Robinson, S. D., Huang, X., Sheppard, D., Hynes, R. O., Hodivala-Dilke, K. M. Enhanced pathological angiogenesis in mice lacking beta-3 integrin or beta-3 and beta-5 integrins. Nature Med. 8: 27-34, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

100 kDa

Applications Notes

6

Gene Name Synonym

Heat shock 105kDa/110kDa protein 1

Subcellular Location

Cytoplasm.

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.