Anti-CYP51A1/CYP51 Antibody Picoband®

About this product

Boster Bio Anti-CYP51A1/CYP51 Antibody Picoband® catalog # A04032-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04032-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-α demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-β-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.

Synonyms

Homeobox protein Hox-A5; Homeobox protein Hox-1C; HOXA5; HOX1C

Gene Name

CYP51A1

Gene ID

1595

UniProt

Q16850

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CYP51A1/CYP51 recombinant protein (Position: K141-K503) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636) . Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636) . Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Applications

WB, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer, Cell Cycle, Cell Cycle Inhibitors, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, p53 Pathway, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.

References & Citations

1. Aldahmesh, M. A., Khan, A. O., Mohamed, J. Y., Hijazi, H., Al-Owain, M., Alswaid, A., Alkuraya, F. S. Genomic analysis of pediatric cataract in Saudi Arabia reveals novel candidate disease genes. Genet. Med. 14: 955-962, 2012. 2. Gillespie, R. L., O'Sullivan, J., Ashworth, J., Bhaskar, S., Williams, S., Biswas, S., Kehdi, E., Ramsden, S. C., Clayton-Smith, J., Black, G. C., Lloyd, I. C. Personalized diagnosis and management of congenital cataract by next-generation sequencing. Ophthalmology 121: 2124-2137, 2014. 3. Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M. S., Madhavan, M., Allan, K. C., Shick, H. E., Garrison, E., Karl, M. T., Factor, D. C., Nevin, Z. S., Sax, J. L. {and 12 others}: Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination. Nature 560: 372-376, 2018.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23310 MW

Observed Molecular Weight

57 kDa

Applications Notes

6

Gene Name Synonym

Homeobox A5

Subcellular Location

Nucleus.

Protein Name

Ran-specific GTPase-activating protein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.