Anti-DCTN1/p150-glued Antibody Picoband®

About this product

Boster Bio Anti-DCTN1/p150-glued Antibody Picoband® catalog # A02175-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02175-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene. This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains ly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA) .

Synonyms

Probetacellulin; Betacellulin; BTC; BTC

Gene Name

DCTN1

Gene ID

1639

UniProt

Q14203

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DCTN1/p150-glued recombinant protein (Position: K1042-S1278) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

References & Citations

1. Caroppo, P., Le Ber, I., Clot, F., Rivaud-Pechoux, S., Camuzat, A., De Septenville, A., Boutoleau-Bretonniere, C., Mourlon, V., Sauvee, M., Lebouvier, T., Bonnet, A.-M., Levy, R., Vercelletto, M., Brice, A. DCTN1 mutation analysis in families with progressive supranuclear palsy-like phenotypes. JAMA Neurol. 71: 208-215, 2014. 2. Collin, G. B., Nishina, P. M., Marshall, J. D., Naggert, J. K. Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome. Genomics 53: 359-364, 1998. 3. Eaton, B. A., Fetter, R. D., Davis, G. W. Dynactin is necessary for synapse stabilization. Neuron 34: 729-741, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63692 MW

Observed Molecular Weight

150 kDa

Applications Notes

6

Gene Name Synonym

Betacellulin

Subcellular Location

Betacellulin: Secreted, extracellular space.

Protein Name

Sentrin-specific protease 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.