Anti-FABP-1/GOT2 Antibody Picoband®

About this product

Boster Bio Anti-FABP-1/GOT2 Antibody Picoband® catalog # A05998-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05998-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aspartate aminotransferase, mitochondrial is an enzyme that in humans is encoded by the GOT2 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Tricarboxylate transport protein, mitochondrial; Citrate transport protein; CTP; Solute carrier family 25 member 1; Tricarboxylate carrier protein; SLC25A1; SLC20A3

Gene Name

GOT2

Gene ID

2806

UniProt

P00505

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FABP-1/GOT2 recombinant protein (Position: H35-K430) .

Clonality

Polyclonal

Tissue Specificity

Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cell Biology, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Citrate transporter that mediates the exchange of mitochondrial citrate for cytosolic malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis- but not trans-aconitate and to a lesser extend maleate and succinate. Important for the bioenergetics of hepatic cells as it provides a carbon source for fatty acid and sterol biosyntheses, and NAD+ for the glycolytic pathway. Required for proper neuromuscular junction formation (Probable) .

References & Citations

1. Andersson, S. M., Pispa, J. P. Purification and properties of human liver tyrosine aminotransferase. Clin. Chim. Acta 125: 117-123, 1982. 2. Barton, D. E., Yang-Feng, T. L., Francke, U. The human tyrosine aminotransferase gene mapped to the long arm of chromosome 16 (region 16q22-q24) by somatic cell hybrid analysis and in situ hybridization. Hum. Genet. 72: 221-224, 1986. 3. Chen, S.-H., Giblett, E. R. Genetic variation of soluble glutamic-oxaloacetic transaminase in man. Am. J. Hum. Genet. 23: 419-424, 1971.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

80420 MW

Observed Molecular Weight

41 kDa

Applications Notes

6

Gene Name Synonym

Solute carrier family 25 member 1

Subcellular Location

Mitochondrion inner membrane. Multi-pass membrane protein.

Protein Name

Long-chain-fatty-acid--CoA ligase 3

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.