Anti-Integrin linked ILK Antibody Picoband®

About this product

Boster Bio Anti-Integrin linked ILK Antibody Picoband® catalog # A02932-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02932-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

Synonyms

Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2

Gene Name

ILK

Gene ID

3611

UniProt

Q13418

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Integrin linked ILK recombinant protein (Position: R9-K452) .

Clonality

Polyclonal

Tissue Specificity

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Applications

ELISA, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction, Transcription, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity) . .

References & Citations

1. Entrez Gene: ILK integrin-linked kinase. 2. Fielding AB, Dobreva I, McDonald PC, Foster LJ, Dedhar S (Feb 2008) . Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. The Journal of Cell Biology 180 (4) : 681–9. 3. Melchior, C., Kreis, S., Janji, B., Kieffer, N. Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1. Biochim. Biophys. Acta 1575: 117-122, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

79686 MW

Observed Molecular Weight

51 kDa

Gene Name Synonym

Enolase 2 (gamma, neuronal)

Subcellular Location

Cytoplasm. Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Protein Name

Peroxisomal multifunctional enzyme type 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.