Anti-PSMB5/MB1 Antibody Picoband®

About this product

Boster Bio Anti-PSMB5/MB1 Antibody Picoband® catalog # A03418-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03418-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proteasome subunit beta type-5 as known as 20S proteasome subunit beta-5 is a protein that in humans is encoded by the PSMB5 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit) . Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Gene Name

PSMB5

Gene ID

5693

UniProt

P28074

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PSMB5/MB1 recombinant protein (Position: M1-P263) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

References & Citations

1. Akiyama, K., Yokota, K., Kagawa, S., Shimbara, N., Tamura, T., Akioka, H., Nothwang, H. G., Noda, C., Tanaka, K., Ichihara, A. cDNA cloning and interferon gamma down-regulation of proteasomal subunits X and Y. Science 265: 1231-1234, 1994. 2. Belich, M. P., Glynne, R. J., Senger, G., Sheer, D., Trowsdale, J. Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteins. Curr. Biol. 4: 769-776, 1994. 3. McCusker, D., Jones, T., Sheer, D., Trowsdale, J. Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex. Genomics 45: 362-367, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

73109 MW

Observed Molecular Weight

22 kDa

Applications Notes

6

Gene Name Synonym

Vacuolar protein sorting 4 homolog B

Subcellular Location

Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.

Protein Name

Interleukin-12 receptor subunit beta-1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.