- Catalog number
- A00061-4
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- C-C chemokine receptor type 5, also known as CCR5 or CD195, is a protein on the surface of white blood cells that is involved in the immune system as it acts as a receptor for chemokines. It is mapped to 3p21.31. This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES) . Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene.
- Synonyms
- C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CHEMR13; HIV-1 fusion coreceptor; CD195; CCR5; CMKBR5
- Gene Name
- CCR5
- Gene ID
- 1234
- UniProt
- P51681
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- A synthetic peptide corresponding to a sequence at C-terminus of human CCR5, which shares 76.2% and 85.7% amino acid (aa) sequence identity with mouse and rat CCR5, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Epressed in mature thymocytes. Detected in monocyte/macrophage cell lines, but not in nonhematopoietic cell lines.
- Applications
- WB
- Field of Research
- Alzheimer's Disease, Antiviral Signaling, Atherosclerosis, Cancer, Cardiovascular, Chemokines, G Protein Signaling, Host-Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interspecies Interaction, Macrophage/Inflammation, Microbiology, Neurodegenerative Disease, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathway, Vascular Inflammation
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.
- References & Citations
- 1. Ahuja, S. K., Kulkarni, H., Catano, G., Agan, B. K., Camargo, J. F., He, W., O'Connell, R. J., Marconi, V. C., Delmar, J., Eron, J., Clark, R. A., Frost, S., Martin, J., Ahuja, S. S., Deeks, S. G., Little, S., Richman, D., Hecht, F. M., Dolan, M. J. CCL3L1-CCR5 genotype influences durability of immune recovery during antiretroviral therapy of HIV-1-infected individuals. Nature Med. 14: 413-420, 2008. 2. Algood, H. M. S., Flynn, J. L. CCR5-deficient mice control Mycobacterium tuberculosis infection despite increased pulmonary lymphocytic infiltration. J. Immun. 173: 3287-3296, 2004. 3. Aliberti, J., Valenzuela, J. G., Carruthers, V. B., Hieny, S., Andersen, J., Charest, H., Reis e Sousa, C., Fairlamb, A., Ribeiro, J. M., Sher, A. Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells. Nature Immun. 4: 485-490, 2003.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 25283 MW
- Observed Molecular Weight
- 41 kDa
- Gene Name Synonym
- C-C motif chemokine receptor 5
- Subcellular Location
- Cell membrane. Multi-pass membrane protein.
- Protein Name
- Tumor necrosis factor receptor superfamily member 6
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
