Anti-LEF1 Antibody Picoband®

About this product

Boster Bio Anti-LEF1 Antibody Picoband® catalog # A00605-2. Tested in ELISA, Flow Cytometry, IF, ICC, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00605-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lymphoid enhancer-binding factor 1 (LEF1) is a protein that in humans is encoded by the LEF1 gene. This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Synonyms

Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; 3.4.24.86; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD156b; ADAM17; CSVP, TACE

Gene Name

LEF1

Gene ID

51176

UniProt

Q9UJU2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human LEF1 recombinant protein (Position: D14-H274) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Proteolytic Enzymes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) . Plays a role in the proteolytic processing of ACE2.

References & Citations

1. de Lau, W., Clevers, H. LEF1 turns over a new leaf. Nature Genet. 28: 3-5, 2001. 2. Driessens, G., Zheng, Y., Gajewski, T. F. Beta-catenin does not regulate memory T cell phenotype. (Letter) Nature Med. 16: 513-514, 2010. 3. Galceran, J., Sustmann, C., Hsu, S.-C., Folberth, S., Grosschedl, R. LEF1-mediated regulation of Delta-like1 links Wnt and Notch signaling in somitogenesis. Genes Dev. 18: 2718-2723, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

58 kDa

Applications Notes

6

Gene Name Synonym

ADAM metallopeptidase domain 17

Subcellular Location

Membrane; Single-pass type I membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.