Anti-ORC5L/ORC5 Antibody Picoband®

About this product

Boster Bio Anti-ORC5L/ORC5 Antibody Picoband® catalog # A03676-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03676-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Synonyms

P-selectin glycoprotein ligand; Selplg; Psgl1

Gene Name

ORC5

Gene ID

5001

UniProt

O43913

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ORC5, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cardiovascular, Cell Biology, Platelets, Protease Inhibitors, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

A SLe (x) -type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

References & Citations

1. Frohling, S., Nakabayashi, K., Scherer, S. W., Dohner, H., Dohner, K. Mutation analysis of the origin recognition complex subunit 5 (ORC5L) gene in adult patients with myeloid leukemias exhibiting deletions of chromosome band 7q22. Hum. Genet. 108: 304-309, 2001. 2. Ishiai, M., Dean, F. B., Okumura, K., Abe, M., Moon, K.-Y., Amin, A. A., Kagotani, K., Taguchi, H., Murakami, Y., Hanaoka, F., O'Donnell, M., Hurwitz, J., Eki, T. Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22. Genomics 46: 294-298, 1997. 3. Quintana, D. G., Thome, K. C., Hou, Z., Ligon, A. H., Morton, C. C., Dutta, A. ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases. J. Biol. Chem. 273: 27137-27145, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28461 MW

Observed Molecular Weight

50 kDa

Applications Notes

6

Gene Name Synonym

Selectin P ligand

Subcellular Location

Integral component of membrane.

Protein Name

Breast cancer metastasis-suppressor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.