Anti-AS160/TBC1D4 Antibody Picoband®

About this product

Boster Bio Anti-AS160/TBC1D4 Antibody Picoband® catalog # A02004-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02004-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

AS160 (Akt substrate of 160 kDa), which was originally known asTBC1 domain family member 4 (TBC1D4), is a RabGTPase-activatingproteinthat in humans is encoded by theTBC1D4gene. This gene is a member of the Tre-2/BUB2/CDC16 domain family. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion.

Synonyms

Bifunctional epoxide hydrolase 2; Cytosolic epoxide hydrolase 2; CEH;3.3.2.10; Epoxide hydratase; Soluble epoxide hydrolase; SEH; Lipid-phosphate phosphatase;3.1.3.76; EPHX2

Gene Name

TBC1D4

Gene ID

9882

UniProt

O60343

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TBC1D4 recombinant protein (Position: K1118-P1298) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. A high level expression is seen in secretory tissues.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cancer Metabolism, Cardiovascular, Cell Biology, Cholesterol Metabolism, Drug Metabolism, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate. .

References & Citations

1. Entrez Gene: TBC1D4 TBC1 domain family, member 4. 2. Kurihara LJ, Semenova E, Miller W, Ingram RS, Guan XJ, Tilghman SM (Feb 2002) . Candidate genes required for embryonic development: a comparative analysis of distal mouse chromosome 14 and human chromosome 13q22.Genomics 79 (2) : 154–61. 3. Sakamoto K, Holman GD (2008) . Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic. Am. J. Physiol. Endocrinol. Metab. 295 (1) : E29–37. doi:10.1152/ajpendo.90331.2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62616 MW

Observed Molecular Weight

160 kDa

Applications Notes

6

Gene Name Synonym

Bifunctional epoxide hydrolase 2

Subcellular Location

Cytoplasm. Peroxisome.

Protein Name

Bifunctional epoxide hydrolase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.