Anti-Hsp90 alpha Antibody Picoband® (monoclonal, 6B5)

About this product

Boster Bio Anti-Hsp90 alpha Antibody Picoband® (monoclonal, 6B5) catalog # M01103-4. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01103-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90-kDa heat shock protein alpha (Hsp90A) . Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.

Synonyms

Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1; KIAA0978

Gene Name

HSP90AA1

Gene ID

3320

UniProt

P07900

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp90 alpha, identical to the related mouse and rat sequences.

Clonality

Monoclonal

Clone

Clone: 6B5

Tissue Specificity

Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, mall intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver.Highly expressed in testes.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

G Protein Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG) .Acts as coactivator of RARA and RXRA through association with NCOA1.Acts as corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner; the function seems to involve differential recruitment of methylated histone H3 to respective promoters.Acts as corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity (By similarity) .Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) .

References & Citations

1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (Dec 2005) . The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics86 (6) : 627–37. 2. Hickey E, Brandon SE, Smale G, Lloyd D, Weber LA (Jun 1989) . Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein. Molecular and Cellular Biology 9 (6) : 2615–26.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

90 kDa

Applications Notes

6

Gene Name Synonym

Additional sex combs like 1, transcriptional regulator

Subcellular Location

Nucleus.

Protein Name

Putative Polycomb group protein ASXL1

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.