Anti-COL4A3 Antibody

About this product

Boster Bio Anti-COL4A3 Antibody Picoband® catalog # A01755-3. Tested in ELISA, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

A01755-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Collagen alpha-3 (IV) chain is a protein that in humans is encoded by the COL4A3 gene. Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

Synonyms

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II; Fp; SDHA; SDH2; SDHF

Gene Name

COL4A3

Gene ID

1285

UniProt

Q01955

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human COL4A3 recombinant protein (Position: P1426-H1670) .

Clonality

Polyclonal

Tissue Specificity

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Applications

IHC, ELISA

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) . Can act as a tumor suppressor.

References & Citations

1. Badenas, C., Praga, M., Tazon, B., Heidet, L., Arrondel, C., Armengol, A., Andres, A., Morales. E., Camacho, J. A., Lens, X., Davila, S., Mila, M., Antignac, C., Darnell, A., Torra, R. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria. J. Am. Soc. Nephrol. 13: 1248-1254, 2002. 2. Bernal, D., Quinones, S., Saus, J. The human mRNA encoding the Goodpasture antigen is alternatively spliced. J. Biol. Chem. 268: 12090-12094, 1993. 3. Butkowski, R. J., Langeveld, J. P. M., Wieslander, J., Hamilton, J., Hudson, B. G. Localization of the Goodpasture epitope to a novel chain of basement membrane collagen. J. Biol. Chem. 262: 7874-7877, 1987.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

130 kDa

Gene Name Synonym

Succinate dehydrogenase complex flavoprotein subunit A

Subcellular Location

Mitochondrion inner membrane. Peripheral membrane protein. Matrix side.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.