Anti-Epac1/RAPGEF3 Antibody Picoband®

About this product

Boster Bio Anti-Epac1/RAPGEF3 Antibody Picoband® catalog # A02483-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02483-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Rap guanine nucleotide exchange factor 3 also known as exchange factor ly activated by cAMP 1 (EPAC1) or cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI) is a protein that in humans is encoded by the RAPGEF3 gene. As the name suggests, EPAC proteins (EPAC1 and EPAC2) are a family of intracellular sensors for cAMP, and function as nucleotide exchange factors for the Rap subfamily of RAS-like small GTPases.

Synonyms

Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP; Squalene- and sterol-carrier protein; SCP; Z-protein; p14; Fabp1

Gene Name

RAPGEF3

Gene ID

10411

UniProt

O95398

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Epac1/RAPGEF3 recombinant protein (Position: H49-R881) .

Clonality

Polyclonal

Tissue Specificity

Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (By similarity) . Binds cholesterol (PubMed:25732850) . Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity) .

References & Citations

1. de Rooij, J., Zwartkruis, F. J. T., Verheijen, M. H. G., Cool, R. H., Nijman, S. M. B., Wittinghofer, A., Bos, J. L. Epac is a Rap1 guanine-nucleotide-exchange factor ly activated by cyclic AMP. Nature 396: 474-477, 1998. 2. Dodge-Kafka, K. L., Soughayer, J., Pare, G. C., Michel, J. J. C., Langeberg, L. K., Kapiloff, M. S., Scott, J. D. The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. Nature 437: 574-578, 2005. 3. Kawasaki, H., Springett, G. M., Mochizuki, N., Toki, S., Nakaya, M., Matsuda, M., Housman, D. E., Graybiel, A. M. A family of cAMP-binding proteins that ly activate Rap1. Science 282: 2275-2279, 1998.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

22105 MW

Observed Molecular Weight

104 kDa

Applications Notes

6

Gene Name Synonym

Fatty acid binding protein 1

Subcellular Location

Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.