Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 8H6)

About this product

Boster Bio Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 8H6) catalog # M01900-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01900-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC) .

Synonyms

Ras-related protein Rab-5A; RAB5A; RAB5

Gene Name

CRYAA

Gene ID

1409

UniProt

P02489

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173) . Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.

Clonality

Monoclonal

Clone

Clone: 8H6

Tissue Specificity

Widely expressed in fetal and adult tissues.

Applications

WB, Flow Cytometry

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience, Neurotransmission, Organelles, Protein Trafficking, Secretory Vesicles, Signal Transduction, Subcellular Markers, Synapse Marker, Tags & Cell Markers, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.

References & Citations

1. Entrez Gene: CRYAA crystallin, alpha A. 2. Derham BK, Harding JJ (1999) . Alpha-crystallin as a molecular chaperone.. Progress in retinal and eye research 18 (4) : 463–509. 3. Jaworski CJ, Piatigorsky J (1989) . A pseudo-exon in the functional human alpha A-crystallin gene.. Nature337 (6209) : 752–4.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

25035 MW

Observed Molecular Weight

20-23 kDa

Applications Notes

6

Gene Name Synonym

RAB5A, member RAS oncogene family

Subcellular Location

Early endosome membrane. Lipid-anchor. Endosome membrane. Cytosol. Cell membrane. Cytoplasmic side. Melanosome. Cytoplasmic vesicle. Ruffle. Membrane. Phagosome membrane.

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.