Anti-DBC-1/CCAR2 Antibody Picoband®

About this product

Boster Bio Anti-DBC-1/CCAR2 Antibody Picoband® catalog # A03412-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03412-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Deleted in bladder cancer protein 1 is a protein that in humans is encoded by the DBC1 gene. Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC) .

Synonyms

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Gene Name

CCAR2

Gene ID

57805

UniProt

Q8N163

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DBC-1/CCAR2 recombinant protein (Position: L65-E917) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) . VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

References & Citations

1. Anantharaman, V., Aravind, L. Analysis of DBC1 and its homologs suggests a potential mechanism for regulation of sirtuin domain deacetylases by NAD metabolites. Cell Cycle 7: 1467-1472, 2008. 2. Close, P., East, P., Dirac-Svejstrup, A. B., Hartmann, H., Heron, M., Maslen, S., Chariot, A., Soding, J., Skehel, M., Svejstrup, J. Q. DBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation. Nature 484: 386-389, 2012. 3. Hamaguchi, M., Meth, J. L., von Klitzing, C., Wei, W., Esposito, D., Rodgers, L., Walsh, T., Welcsh, P., King, M.-C., Wigler, M. H. DBC2, a candidate for a tumor suppressor gene involved in breast cancer. Proc. Nat. Acad. Sci. 99: 13647-13652, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

73109 MW

Observed Molecular Weight

130 kDa

Applications Notes

6

Gene Name Synonym

Vacuolar protein sorting 4 homolog B

Subcellular Location

Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.

Protein Name

Interleukin-12 receptor subunit beta-1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.