Anti-GCKR Antibody Picoband®

About this product

Boster Bio Anti-GCKR Antibody Picoband® catalog # A02122-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02122-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The glucokinase regulatory protein (GKRP) also known as glucokinase (hexokinase 4) regulator (GCKR) is a protein produced in hepatocytes (liver cells) . This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY) .

Synonyms

Carnitine O-palmitoyltransferase 2, mitochondrial; Carnitine palmitoyltransferase II; CPT II; CPT2; CPT1

Gene Name

GCKR

Gene ID

2646

UniProt

Q14397

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GCKR recombinant protein (Position: Q8-Q569) .

Clonality

Polyclonal

Tissue Specificity

Expressed mainly in adipose tissues.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Cardiovascular, G Protein Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.

References & Citations

1. Beer, N. L., Tribble, N. D., McCulloch, L. J., Roos, C., Johnson, P. R. V., Orho-Melander, M., Gloyn, A. L. The P446L variant in GCKR associated with fasting plasma glucose and triglyceride levels exerts its effect through increased glucokinase activity in liver. Hum. Molec. Genet. 18: 4081-4088, 2009. 2. Dupuis, J., Langenberg, C., Prokopenko, I., Saxena, R., Soranzo, N., Jackson, A. U., Wheeler, E., Glazer, N. L., Bouatia-Naji, N., Gloyn, A. L., Lindgren, C. M., Magi, R., and 295 others. New genetic loci implicated in fasting glucose homeostasis and their impact on type 2 diabetes risk. Nature Genet. 42: 105-116, 2010. Note: Erratum: Nature Genet. 42: 464 only, 2010. 3. Farrelly, D., Brown, K. S., Tieman, A., Ren, J., Lira, S. A., Hagan, D., Gregg, R., Mookhtiar, K. A., Hariharan, N. Mice mutant for glucokinase regulatory protein exhibit decreased liver glucokinase: a sequestration mechanism in metabolic regulation. Proc. Nat. Acad. Sci. 96: 14511-14516, 1999.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

51990 MW

Observed Molecular Weight

69 kDa

Applications Notes

6

Gene Name Synonym

Carnitine palmitoyltransferase 2

Subcellular Location

Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side

Protein Name

Angiotensinogen

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.