Anti-RPA32/RPA2 Antibody Picoband® (monoclonal, 3B2E9)

About this product

Boster Bio Anti-RPA32/RPA2 Antibody Picoband® (monoclonal, 3B2E9) catalog # M02067-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02067-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Synonyms

Desmin; DES

Gene Name

RPA2

Gene ID

6118

UniProt

P15927

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RPA32/RPA2 recombinant protein (Position: Q34-H254) .

Clonality

Monoclonal

Clone

Clone: 3B2E9

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division,2339, Chromosome Structure, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.

References & Citations

1. Chaudhuri, J., Khuong, C., Alt, F. W. Replication protein A interacts with AID to promote deamination of somatic hypermutation targets. Nature 430: 992-998, 2004. 2. Lee, D.-H., Pan, Y., Kanner, S., Sung, P., Borowiec, J. A., Chowdhury, D. A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Nature Struct. Molec. Biol. 17: 365-372, 2010. 3. Umbricht, C. B., Griffin, C. A., Hawkins, A. L., Grzeschik, K. H., O'Connell, P., Leach, R., Green, E. D., Kelly, T. J. High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3) . Genomics 20: 249-257, 1994.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

32 kDa

Applications Notes

6

Gene Name Synonym

Desmin

Subcellular Location

Nucleus. Sarcolemma. Z line. Cytoplasm.

Isotype

Mouse IgG2a

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.