Anti-Cyclophilin E/PPIE Antibody Picoband® (monoclonal, 4I9)

About this product

Boster Bio Anti-Cyclophilin E/PPIE Antibody Picoband® (monoclonal, 4I9) catalog # M08021. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M08021

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

Synonyms

T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5

Gene Name

PPIE

Gene ID

10450

UniProt

Q9UNP9

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301) .

Clonality

Monoclonal

Clone

Clone: 4I9

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

References & Citations

1. Kim, J.-O., Nau, M. M., Allikian, K. A., Makela, T. P., Alitalo, K., Johnson, B. E., Kelley, M. J. Co-amplification of a novel cyclophilin-like gene (PPIE) with L-myc in small cell lung cancer cell lines. Oncogene 17: 1019-1026, 1998. 2. Mi, H., Kops, O., Zimmermann, E., Jaschke, A., Tropschug, M. A nuclear RNA-binding cyclophilin in human T cells. FEBS Lett. 398: 201-205, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

35 kDa

Applications Notes

6

Gene Name Synonym

Chaperonin containing TCP1 subunit 3

Subcellular Location

Cytoplasm.

Isotype

Mouse IgG2b

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.