- Catalog number
- A00456-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) . In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
- Gene Name
- B2M
- Gene ID
- 567
- UniProt
- P61769
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119) . Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Detected in lung, spleen, uterus, vagina and epididymis. .
- Applications
- ELISA (Cap), Flow Cytometry, IF, IHC, ICC, WB
- Field of Research
- Immunoglobulins, Immunology
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .
- References & Citations
- 1. Güssow D, Rein R, Ginjaar I, Hochstenbach F, Seemann G, Kottman A, Ploegh HL (1 November 1987) . The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit. J. Immunol. 139 (9) : 3132–8. 2. Munshi NC, Longo DL, Anderson KC (2011) . Chapter 111: Plasma Cell Disorders. In Loscalzo J, Longo DL, Fauci AS, Dennis LK, Hauser SL. Harrison's Principles of Internal Medicine (18th ed.) . McGraw-Hill Professional. pp. 936–44. 3. Pignone M, Nicoll D; McPhee SJ (2004) . Pocket guide to diagnostic tests (4th ed.) . New York: McGraw-Hill. p. 191. ISBN 0-07-141184-4.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 22875 MW
- Observed Molecular Weight
- 14 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- Neutrophil gelatinase-associated lipocalin
- Subcellular Location
- Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Protein Name
- Neutrophil gelatinase-associated lipocalin
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
