Anti-AGO1 Antibody Picoband®

About this product

Boster Bio Anti-AGO1 Antibody Picoband® catalog # A00826-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00826-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Synonyms

Protein argonaute-1; Argonaute1; hAgo1; Argonaute RISC catalytic component 1; Eukaryotic translation initiation factor 2C 1; eIF-2C 1; eIF2C 1; Putative RNA-binding protein Q99; AGO1; EIF2C1

Gene Name

AGO1

Gene ID

26523

UniProt

Q9UL18

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human AGO1 recombinant protein (Position: E376-R409) .

Clonality

Polyclonal

Tissue Specificity

Detected in blood plasma (at protein level) .

Applications

WB, Flow Cytometry, ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Division, Cytokinesis, DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for RNA-mediated gene silencing (RNAi) . Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs) . .

References & Citations

1. Guo, S.; Kemphues, K.J. (1995) . par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell. 81 (4) : 611–620. 2. Hannon, GJ (2002) . RNA interference. Nature.418 (6894) : 244–51. 3. Meins F, Jr; Si-Ammour, A; Blevins, T (2005) . RNA silencing systems and their relevance to plant development.. Annual Review of Cell and Developmental Biology. 21 (1) : 297–318.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

97214 MW

Observed Molecular Weight

97 kDa

Applications Notes

6

Gene Name Synonym

Protein argonaute-1

Subcellular Location

Cytoplasm, P-body .

Protein Name

Protein argonaute-1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.