Anti-ATP1B1 Antibody Picoband®

About this product

Boster Bio Anti-ATP1B1 Antibody Picoband® catalog # A03469-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03469-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sodium/potassium-transporting ATPase subunit beta-1 is an enzyme that in humans is encoded by the ATP1B1 gene. The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta) . The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.

Synonyms

Methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4; MED1

Gene Name

ATP1B1

Gene ID

481

UniProt

P05026

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ATP1B1 recombinant protein (Position: Q55-E271) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

References & Citations

1. Chang, Y.-P. C., Liu, X., Kim, J. D. O., Ikeda, M. A., Layton, M. R., Weder, A. B., Cooper, R. S., Kardia, S. L. R., Rao, D. C., Hunt, S. C., Luke, A., Boerwinkle, E., Chakravarti, A. Multiple genes for essential-hypertension susceptibility on chromosome 1q. Am. J. Hum. Genet. 80: 253-264, 2007. 2. Kawakami, K., Nojima, H., Ohta, T., Nagano, K. Molecular cloning and sequence analysis of human Na, K-ATPase beta-subunit. Nucleic Acids Res. 14: 2833-2844, 1986. 3. Kent, R. B., Fallows, D. A., Geissler, E., Glaser, T., Emanuel, J. R., Lalley, P. A., Levenson, R., Housman, D. E. Genes encoding alpha and beta subunits of Na, K-ATPase are located on three different chromosomes in the mouse. Proc. Nat. Acad. Sci. 84: 5369-5373, 1987.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

50 kDa

Applications Notes

6

Gene Name Synonym

Methyl-CpG binding domain 4, DNA glycosylase

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.